The analysis of triacylglycerols by gas chromatography (GC) in their native state is challenging, largely due to their poor volatility and thermal decomposition in hot injector ports. To improve their properties for GC and to provide a comprehensive characterization of fatty acids, the esterified fatty acyl chains must be cleaved from the glycerol backbone and methylated to derive fatty acid methyl esters (FAMEs). This application note reports the results from the analysis of FAMEs from edible oils performed with the PerkinElmer GC 2400 System equipped with capillary injector, Flame Ionization Detector (FID), and Elite-2560 column showing fast analysis time and high reproducibility.